The first Piccadilly line train was built in Vienna and is undergoing testing by engineers in Germany.
The trains have been designed to improve accessibility and are more energy efficient, the company said.
Stuart Harvey, the chief capital officer at Transport for London (TfL), said: “Producing more Piccadilly line trains in Goole will support local supply chains, clearly demonstrating how investment in London benefits the whole of the UK.”
Officials at TfL also said the Goole factory would produce a new fleet for the Bakerloo line once government funding is confirmed.
The rail village includes a service facility, logistics warehouse and development hub.
Siemens Mobility said it would establish the area as a centre of excellence for UK rail technology, creating up to 700 jobs. A further 1,700 jobs will be created in the supply chain, it added.